Journal:

Article Title: Constitutive Expression of a Chromosomal Class A (BJM Group 2) ?-Lactamase in Xanthomonas campestris

doi: 10.1128/AAC.48.1.209-215.2004

Figure Lengend Snippet: Bacterial strains used in this study

Article Snippet: Antibiotics were added when necessary: ampicillin (50 μg/ml), kanamycin (50 μg/ml), and tetracycline (15 μg/ml). table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain Characteristic(s) Source or reference E. coli DH5α endA1 hsdR17 (r k − m k + ) supE44 thi-1 recA gyrA relA1 φ80d lacZ ΔM15 Δ( lacZYA - argF ) Bethesda Research Laboratories ATCC 25922 Reference strain for MIC tests American Type Culture Collection E. coli (pFY13-9BSRX) DH5α with plasmid pFY13-9BSRX carrying the entire ampR1 and bla XCC-1 genes, Km r /Ap s This study E. coli (pBX) DH5α with plasmid pBX carrying the bla XCC-1 promoter situated upstream of the luxA and fluxB reporter genes, Tc r /Ap s This study E. coli (pRX) DH5α with plasmid pRX carrying the ampR1 promoter situated upstream of the luxA and luxB reporter genes, Tc r /Ap s This study X. campestris pv. campestris Xc2 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection a Xc6 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection Xc85 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection Xc17 Wild-type strain isolated in Taiwan, Ap r Yang and Tseng ( 33 ) Xc11 Wild-type strain isolated in Taiwan, Ap r Yang and Tseng ( 33 ) Xc11A Spontaneous avirulent mutant from Xc11, Ap r Yang and Tseng ( 33 ) Xc11bla::Tc Xc11 bla XCC-1 mutant constructed by inserting a Tc r cartridge into the Sma I site of the bla XCC-1 gene, Tc r /Ap s Weng et al. ( 29 ) Xc11RM Xc11 ampR1 mutant constructed by inserting a Tc r cartridge into the Sal I site of the ampR1 gene, Tc r /Ap s This study Xc11bla::Tc(pRKSE) Xc11 bla XCC-1 mutant complemented with cloned bla XCC-1 gene, Tc r /Ap r /Km r Weng et al. ( 29 ) Xc11RM(pFJ-ampR1) Xc11 ampR1 mutant complemented with cloned ampR1 gene, Tc r /Ap r /Km r This study X. campestris pv. begoniae 59 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection X. campestris pv. dieffenbachiae 65 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection X. campestris pv. mangiferaeindcae 38 Wild-type strain isolated in Taiwan, Ap s Tseng lab collection X. campestris pv. phaseoli 73 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection X. axonopodis pv. citri 60 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection X. axonopodis pv. glycines 69 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection X. oryzae pv. oryzae 21 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection X. axonopodis pv. vesicatoria Xv64 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection Xv3240 European strain, Ap s Ojanen et al. ( 20 ) Serratia marcescens 6 Wild-type strain isolated in Taiwan, Ap r L. T. Wu, China Medical University Open in a separate window a Yi-Hsiung Tseng's laboratory collection.

Techniques: Plasmid Preparation, Isolation, Mutagenesis, Construct, Clone Assay

Journal:

Article Title: Constitutive Expression of a Chromosomal Class A (BJM Group 2) ?-Lactamase in Xanthomonas campestris

doi: 10.1128/AAC.48.1.209-215.2004

Figure Lengend Snippet: Inserts of the plasmids carrying blaXCC-1 and/or ampR1 from X. campestris pv. campestris strain 11. The 4.0-kb Sau3A1 fragment in pCH1, the 2.5-kb StuI-XbaI fragment in pFY13-9BSRX, the 1.7-kb EcoRI-SstI fragment in pBKE, the 0.5-kb PstI-SstI fragment in pBKX, the 1.0-kb SmaI-SstI fragment in pOKSS, the 1.3-kb PstI-XbaI fragment in pFJ-ampR1, the 0.5-kb PstI-PvuII fragment in pBX, and the same fragment in the opposite orientation in pRX are shown. The underlined restriction sites are from the multiple cloning sites of the vectors.

Article Snippet: Antibiotics were added when necessary: ampicillin (50 μg/ml), kanamycin (50 μg/ml), and tetracycline (15 μg/ml). table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain Characteristic(s) Source or reference E. coli DH5α endA1 hsdR17 (r k − m k + ) supE44 thi-1 recA gyrA relA1 φ80d lacZ ΔM15 Δ( lacZYA - argF ) Bethesda Research Laboratories ATCC 25922 Reference strain for MIC tests American Type Culture Collection E. coli (pFY13-9BSRX) DH5α with plasmid pFY13-9BSRX carrying the entire ampR1 and bla XCC-1 genes, Km r /Ap s This study E. coli (pBX) DH5α with plasmid pBX carrying the bla XCC-1 promoter situated upstream of the luxA and fluxB reporter genes, Tc r /Ap s This study E. coli (pRX) DH5α with plasmid pRX carrying the ampR1 promoter situated upstream of the luxA and luxB reporter genes, Tc r /Ap s This study X. campestris pv. campestris Xc2 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection a Xc6 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection Xc85 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection Xc17 Wild-type strain isolated in Taiwan, Ap r Yang and Tseng ( 33 ) Xc11 Wild-type strain isolated in Taiwan, Ap r Yang and Tseng ( 33 ) Xc11A Spontaneous avirulent mutant from Xc11, Ap r Yang and Tseng ( 33 ) Xc11bla::Tc Xc11 bla XCC-1 mutant constructed by inserting a Tc r cartridge into the Sma I site of the bla XCC-1 gene, Tc r /Ap s Weng et al. ( 29 ) Xc11RM Xc11 ampR1 mutant constructed by inserting a Tc r cartridge into the Sal I site of the ampR1 gene, Tc r /Ap s This study Xc11bla::Tc(pRKSE) Xc11 bla XCC-1 mutant complemented with cloned bla XCC-1 gene, Tc r /Ap r /Km r Weng et al. ( 29 ) Xc11RM(pFJ-ampR1) Xc11 ampR1 mutant complemented with cloned ampR1 gene, Tc r /Ap r /Km r This study X. campestris pv. begoniae 59 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection X. campestris pv. dieffenbachiae 65 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection X. campestris pv. mangiferaeindcae 38 Wild-type strain isolated in Taiwan, Ap s Tseng lab collection X. campestris pv. phaseoli 73 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection X. axonopodis pv. citri 60 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection X. axonopodis pv. glycines 69 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection X. oryzae pv. oryzae 21 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection X. axonopodis pv. vesicatoria Xv64 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection Xv3240 European strain, Ap s Ojanen et al. ( 20 ) Serratia marcescens 6 Wild-type strain isolated in Taiwan, Ap r L. T. Wu, China Medical University Open in a separate window a Yi-Hsiung Tseng's laboratory collection.

Techniques: Clone Assay